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Journal Articles Molecular Microbiology Year : 2022

Overexpression approaches to advance understanding of Candida albicans

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Abstract

Candida albicans is an opportunistic fungal pathogen that is responsible for infections linked to high mortality. Loss-of-function approaches, taking advantage of gene knockouts or inducible down-regulation, have been successfully used in this species in order to understand gene function. However, overexpression of a gene provides an alternative, powerful tool to elucidate gene function and identify novel phenotypes. Notably, overexpression can identify pathway components that might remain undetected using loss-of-function approaches. Several repressible or inducible promoters have been developed which allow to shut off or turn on the expression of a gene in C. albicans upon growth in the presence of a repressor or inducer. In this review, we summarize recent overexpression approaches used to study different aspects of C. albicans biology, including morphogenesis, biofilm formation, drug tolerance, and commensalism.
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Dates and versions

hal-03755737 , version 1 (22-08-2022)

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Attribution - CC BY 4.0

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Laxmi Shanker Rai, Lasse Wijlick, Murielle Chauvel, Christophe D'Enfert, Mélanie Legrand, et al.. Overexpression approaches to advance understanding of Candida albicans. Molecular Microbiology, 2022, 117 (3), pp.589-599. ⟨10.1111/mmi.14818⟩. ⟨hal-03755737⟩
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