Pedigree reconstruction for triploid apple cultivars using single nucleotide polymorphism array data - Archive ouverte HAL Access content directly
Journal Articles Plants, People, Planet Year : 2022

Pedigree reconstruction for triploid apple cultivars using single nucleotide polymorphism array data

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Abstract

In apple (Malus domestica), most cultivars are diploid, though a sizeable number are triploids, which tend to be stronger growing, more robust, and bear larger fruit.However, triploidy is also associated with strongly reduced fertility. Some recorded pedigrees for historical apple cultivars include triploids as parents of dip-loids, despite this reputation of poor fertility. This information, coupled with some initiatives using triploids in breeding efforts, result in confusion about how possible or common it is for triploids to be parents of diploid off spring. To date, no studies have systematically evaluated and identified pedigrees of triploid apple cultivars to resolve these contradictions. •Here, we describe a method to make triploid genotype calls using Illumina Infinium single nucleotide polymorphism (SNP) array data through a novel Python script:ploidy Classifier. SNP data for 219 unique triploids was compared alongside 2498unique diploid apple accessions to conduct pedigree reconstruction. •Unreduced gamete-donating parents were identified for over half of the triploid accessions. From those, reduced gamete-donating parents were identified fornearly half. Full or partial pedigrees for many classic triploids were uncovered, including that of the oldest known American cultivar, ‘Roxbury Russet’. All tested pedigrees from literature that listed triploids as parents of diploids were deemed false, including that of the well-known‘Cox's Orange Pippin’, whose previously unreported second parent was also identified here as‘Rosemary Russet’ .•These results together suggest that historic triploids are mostly or solely the prod-uct of diploid parentage and that triploidy has been a dead end in historic apple pedigrees.
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Dates and versions

hal-03761410 , version 1 (15-09-2022)

Licence

Attribution - CC BY 4.0

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Nicholas Howard, Diego Micheletti, James Luby, Charles‐eric Durel, Caroline Denancé, et al.. Pedigree reconstruction for triploid apple cultivars using single nucleotide polymorphism array data. Plants, People, Planet, 2022, early access, ppp3.10313. ⟨10.1002/ppp3.10313⟩. ⟨hal-03761410⟩
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