Superoxide anion scavenging capacity measured by a polarographic method. Comparison with a colourimetric method - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Free Radical Research Communications Year : 2009

Superoxide anion scavenging capacity measured by a polarographic method. Comparison with a colourimetric method

Abstract

A polarographic method to assess the scavenging capacity of a molecule for O2- is proposed. This method is based on the fact that O2- is not detected by the Clark electrode and that a scavenger competes with spontaneous dismutation of O2-. So, the reduction of O2 into O2- and the decomposition of H2O2 by catalase, releasing O2, show a biphasic kinetic. Various kinetic parameters can be used to calculate the nmol of O2- scavenged and also supply data on the reaction mechanisms (oxidation or reduction of O2-) involved in scavenging. This method presents several other advantages: scavenging capacity can be assayed without added indicators which themselves behave as scavengers (as demonstrated for NBT), the presence of scavengers which interfere with the O2- generating system (xanthine-xanthine oxidase) does not invalidate the measurements made.
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Dates and versions

hal-03761458 , version 1 (26-08-2022)

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Nicole Darmon, Y. Fernandez, A. Periquet, S. Mitjavila. Superoxide anion scavenging capacity measured by a polarographic method. Comparison with a colourimetric method. Free Radical Research Communications, 2009, 17 (2), pp.97-107. ⟨10.3109/10715769209082267⟩. ⟨hal-03761458⟩
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