Antimicrobial and cytotoxic effects of marine sponge extracts <i>Agelas clathrodes</i>, <i>Desmapsamma anchorata</i> and <i>Verongula rigida</i> from a Caribbean Island - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue PeerJ Année : 2022

Antimicrobial and cytotoxic effects of marine sponge extracts Agelas clathrodes, Desmapsamma anchorata and Verongula rigida from a Caribbean Island

Julie Piron
  • Fonction : Auteur
Niklas Tysklind
Juliette Smith-Ravin
  • Fonction : Auteur
  • PersonId : 1027425
Fabienne Priam
  • Fonction : Auteur
  • PersonId : 1025447

Résumé

Although marine sponges are known for their antimicrobial, antifungal and cytotoxic activity, very few studies have been carried out on endemic species of Martinique. Martinique is part of the Agoa Sanctuary, a marine protected area that includes the exclusive economic zones (EEZ) of the French Caribbean islands, making it an abundant source of marine species. To highlight the potential of this area for the discovery of marine biomolecules with antipathogenic and antitumor activities, we tested the aqueous and ethanolic extracts of sponge species Agelas clathrodes, Desmapsamma anchorata and Verongula rigida. Five bacterial strains: Bacillus cereus (CIP 78.3), Escherichia coli (CIP 54.127), Pseudomonas aeruginosa (CIP A22), Staphylococcus aureus (CIP 67.8) and Staphylococcus saprophyticus (CIP 76125) were evaluated, as well as four tumor cell lines: breast cancer (MDA-MB231), glioblastoma (RES259) and leukemia (MOLM14 and HL-60). Antimicrobial activity was evaluated using the disc diffusion technique by determining the minimum inhibitory and minimum bactericidal concentrations. Tumor cytotoxic activity was determined in vitro by defining the minimum concentration of extracts that would inhibit cell growth. Ethanolic extracts of Agelas clathrodes were bactericidal for Staphylococcus aureus and Staphylococcus saprophyticus strains, as well as strongly cytotoxic (IC 50 < 20 µg/mL) on all cancer cell lines. Verongula rigida also showed strong cytotoxic activity on cell lines but no antimicrobial activity. These results are innovative for this species on these bacterial lines, highlighting the potential of sponge extracts from this area as bioactive compounds sources.
Fichier principal
Vignette du fichier
Piron et al, 2022 (antimicrobial and cytotoxic effects of marine sponge extracts).pdf (2.41 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Licence : CC BY - Paternité

Dates et versions

hal-03803766 , version 1 (06-10-2022)

Licence

Paternité

Identifiants

Citer

Julie Piron, Stéphane Betzi, Jessica Pastour, Audrey Restouin, Remy Castellano, et al.. Antimicrobial and cytotoxic effects of marine sponge extracts Agelas clathrodes, Desmapsamma anchorata and Verongula rigida from a Caribbean Island. PeerJ, 2022, 10, pp.e13955. ⟨10.7717/peerj.13955⟩. ⟨hal-03803766⟩
79 Consultations
33 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More