Abstract Stringent discharge regulations are encouraging researchers to create innovative and sustainable wastewater treatment solutions. Urine source separation (USS) is among the potent approaches that may reduce nutrient peak loads in the influent wastewater and improve nutrient recovery. A phenomenological model was used to simulate dynamic influent properties and predict the advantages gained from implementing USS in an urban water basin. Several scenarios were investigated assuming different levels of deployment: at the entire city, or specifically in office buildings for men's urine only, or for both men and women employees. The results confirmed that all scenarios of urine source separation offered benefits at the treatment plant in terms of reducing nitrogen influent load. The economic benefits in terms of reducing energy consumption for nitrification and decreasing methanol addition for denitrification were quantified, and results confirmed environmental advantages gained from different USS scenarios. Despite larger advantages gained from a global USS rate in an entire city, implementation of a specific USS in office buildings would remain more feasible from a logistical perspective. A significant benefit in terms of reducing greenhouse gas emissions is demonstrated and this was especially due to the high level of N2O emissions avoided in nitrifying biological aerated filter.