Maintenance of divergent lineages of the Rice Blast Fungus Pyricularia oryzae through niche separation, loss of sex and post-mating genetic incompatibilities - Archive ouverte HAL Access content directly
Journal Articles PLoS Pathogens Year : 2022

Maintenance of divergent lineages of the Rice Blast Fungus Pyricularia oryzae through niche separation, loss of sex and post-mating genetic incompatibilities

(1, 2) , (3, 4) , (1) , (1, 4) , (1, 4) , (1) , (1) , (2) , (1) , (2) , (1) , (1, 4) , (1)
1
2
3
4

Abstract

Many species of fungal plant pathogens coexist as multiple lineages on the same host, but the factors underlying the origin and maintenance of population structure remain largely unknown. The rice blast fungus Pyricularia oryzae is a widespread model plant pathogen displaying population subdivision. However, most studies of natural variation in P. oryzae have been limited in genomic or geographic resolution, and host adaptation is the only factor that has been investigated extensively as a contributor to population subdivision. In an effort to complement previous studies, we analyzed genetic and phenotypic diversity in isolates of the rice blast fungus covering a broad geographical range. Using single-nucleotide polymorphism genotyping data for 886 isolates sampled from 152 sites in 51 countries, we showed that population subdivision of P. oryzae in one recombining and three clonal lineages with broad distributions persisted with deeper sampling. We also extended previous findings by showing further population subdivision of the recombining lineage into one international and three Asian clusters, and by providing evidence that the three clonal lineages of P. oryzae were found in areas with different prevailing environmental conditions, indicating niche separation. Pathogenicity tests and bioinformatic analyses using an extended set of isolates and rice varieties indicated that partial specialization to rice subgroups contributed to niche separation between lineages, and differences in repertoires of putative virulence effectors were consistent with differences in host range. Experimental crosses revealed that female sterility and early post-mating genetic incompatibilities acted as strong additional barriers to gene flow between clonal lineages. Our results demonstrate that the spread of a fungal pathogen across heterogeneous habitats and divergent populations of a crop species can lead to niche separation and reproductive isolation between distinct, widely distributed, lineages.
Fichier principal
Vignette du fichier
Thierry-PP-2022-CC-BY.pdf (3.57 Mo) Télécharger le fichier
Vignette du fichier
Thierry-PP-2022-CC-BY (1).pdf (3.57 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive
Licence : CC BY - Attribution

Dates and versions

hal-03948351 , version 1 (20-01-2023)

Licence

Attribution - CC BY 4.0

Identifiers

Cite

Maud Thierry, Florian Charriat, Joëlle Milazzo, Henri Adreit, Sébastien Ravel, et al.. Maintenance of divergent lineages of the Rice Blast Fungus Pyricularia oryzae through niche separation, loss of sex and post-mating genetic incompatibilities. PLoS Pathogens, 2022, 18, pp.e1010687. ⟨10.1371/journal.ppat.1010687⟩. ⟨hal-03948351⟩
0 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More