Cereal-legume mixture is a well-known successful intercrop model for an efficient use of soil nutrients [1 , 2]. Effects of mineral N gradient on the acquisition of major nutrients: potassium (K), calcium (Ca), magnesium (Mg) and sulfur (S) is presented. A greenhouse pot experiment was conducted with wheat (Triticum aestivum L. cv. Lennox) and white lupin (Lupinus albus L. cv. Feodora) grown as sole crops and intercropped along a soil mineral N gradient obtained by 15 N addition. Plants were harvested at flowering stage and dry weights of shoots and roots were measured. Potassium, calcium, magnesium and sulfur concentrations in shoots and roots were determined by Inductively Coupled Plasma Mass Spectrometry (ICP-MS).