Divergence of a genomic island leads to the evolution of melanization in a halophyte root fungus - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles The International Society of Microbiologial Ecology Journal Year : 2021

Divergence of a genomic island leads to the evolution of melanization in a halophyte root fungus

Zhilin Yuan
Irina Druzhinina
  • Function : Author
John Gibbons
Zhenhui Zhong
Yves van de Peer
  • Function : Author
Russell Rodriguez
  • Function : Author
Zhongjian Liu
Xinyu Wang
Huanshen Wei
Qi Wu
  • Function : Author
Jieyu Wang
  • Function : Author
Guohui Shi
Feng Cai
Long Peng
Francis Martin

Abstract

Abstract Understanding how organisms adapt to extreme living conditions is central to evolutionary biology. Dark septate endophytes (DSEs) constitute an important component of the root mycobiome and they are often able to alleviate host abiotic stresses. Here, we investigated the molecular mechanisms underlying the beneficial association between the DSE Laburnicola rhizohalophila and its host, the native halophyte Suaeda salsa , using population genomics. Based on genome-wide Fst (pairwise fixation index) and Vst analyses, which compared the variance in allele frequencies of single-nucleotide polymorphisms (SNPs) and copy number variants (CNVs), respectively, we found a high level of genetic differentiation between two populations. CNV patterns revealed population-specific expansions and contractions. Interestingly, we identified a ~20 kbp genomic island of high divergence with a strong sign of positive selection. This region contains a melanin-biosynthetic polyketide synthase gene cluster linked to six additional genes likely involved in biosynthesis, membrane trafficking, regulation, and localization of melanin. Differences in growth yield and melanin biosynthesis between the two populations grown under 2% NaCl stress suggested that this genomic island contributes to the observed differences in melanin accumulation. Our findings provide a better understanding of the genetic and evolutionary mechanisms underlying the adaptation to saline conditions of the L. rhizohalophila–S. salsa symbiosis.
Fichier principal
Vignette du fichier
2021_Yuan_ISME J.pdf (3.02 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive
Licence : CC BY - Attribution

Dates and versions

hal-04000960 , version 1 (22-02-2023)

Licence

Attribution

Identifiers

Cite

Zhilin Yuan, Irina Druzhinina, John Gibbons, Zhenhui Zhong, Yves van de Peer, et al.. Divergence of a genomic island leads to the evolution of melanization in a halophyte root fungus. The International Society of Microbiologial Ecology Journal, 2021, 15 (12), pp.3468-3479. ⟨10.1038/s41396-021-01023-8⟩. ⟨hal-04000960⟩
8 View
5 Download

Altmetric

Share

Gmail Facebook X LinkedIn More