Novel Ehrlichia canis genogroup in dogs with canine ehrlichiosis in Cuba - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Parasites & Vectors Year : 2022

Novel Ehrlichia canis genogroup in dogs with canine ehrlichiosis in Cuba

Maylin González Navarrete
  • Function : Author
Adnan Hodžić
  • Function : Author
Belkis Corona-González
  • Function : Author
Matheus Dias Cordeiro
  • Function : Author
Claudia Bezerra da Silva
  • Function : Author
Liani Coronado Báez
  • Function : Author
Dasiel Obregón
  • Function : Author
Daniel Moura de Aguiar
  • Function : Author
Amanda Noeli da Silva Campos
  • Function : Author
Alejandra Wu-Chuang
Eugenio Roque López
  • Function : Author
Elianne Piloto-Sardiñas
  • Function : Author
Lianet Abuin-Denis
  • Function : Author
Adivaldo Henrique da Fonseca
  • Function : Author

Abstract

Abstract Background Canine monocytic ehrlichiosis (CME) is caused by the tick-borne pathogen Ehrlichia canis , an obligate intracellular Gram-negative bacterium of the family Anaplasmataceae with tropism for canine monocytes and macrophages. The trp36 gene, which encodes for the major immunoreactive protein TRP36 in E. canis , has been successfully used to characterize the genetic diversity of this pathogen in different regions of the world. Based on trp36 sequence analysis, four E. canis genogroups, United States (US), Taiwan (TWN), Brazil (BR) and Costa Rica (CR), have been identified. The aim of this study was to characterize the genetic diversity of E. canis in Cuba based on the trp36 gene. Methods Whole blood samples ( n = 8) were collected from dogs found to be infested with the tick vector Rhipicephalus sanguineus sensu lato (s.l.) and/or presenting clinical signs and symptoms of CME. Total DNA was extracted from the blood samples and trp36 fragments were amplified by PCR. Nucleotide and protein sequences were compared using alignments and phylogenetic analysis. Results Four of the trp36 sequences obtained ( n = 8) fall within the phylogenetic cluster grouping the US genogroup E. canis strains. The other E. canis trp36 sequences formed a separate and well-supported clade (94% bootstrap value) that is phylogenetically distant from the other major groups and thus represents a new genogroup, herein designated as the ‘Cuba (CUB) genogroup’. Notably, dogs infected with the CUB genogroup presented frequent hemorrhagic lesions. Conclusions The results of this study suggest that genetic diversification of E. canis in Cuba is associated with the emergence of E. canis strains with increased virulence.
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hal-04009215 , version 1 (01-03-2023)

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Maylin González Navarrete, Adnan Hodžić, Belkis Corona-González, Matheus Dias Cordeiro, Claudia Bezerra da Silva, et al.. Novel Ehrlichia canis genogroup in dogs with canine ehrlichiosis in Cuba. Parasites & Vectors, 2022, 15 (1), pp.295. ⟨10.1186/s13071-022-05426-0⟩. ⟨hal-04009215⟩
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