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Article Dans Une Revue Foods Année : 2023

Ionic Strength Dependence of the Complex Coacervation between Lactoferrin and β-Lactoglobulin

Résumé

Heteroprotein complex coacervation is an assembly formed by oppositely charged proteins in aqueous solution that leads to liquid-liquid phase separation. The ability of lactoferrin and βlactoglobulin to form complex coacervates at pH 5.5 under optimal protein stoichiometry has been studied in a previous work. The goal of the current study is to determine the influence of ionic strength on the complex coacervation between these two proteins using direct mixing and desalting protocols. The initial interaction between lactoferrin and β-lactoglobulin and subsequent coacervation process were highly sensitive to the ionic strength. No microscopic phase separation was observed beyond a salt concentration of 20 mM. The coacervate yield decreased drastically with increasing added NaCl from 0 to 60 mM. The charge-screening effect induced by increasing the ionic strength is attributed to a decrease of interaction between the two oppositely charged proteins throughout a decrease in Debye length. Interestingly, as shown by isothermal titration calorimetry, a small concentration of NaCl around 2.5 mM promoted the binding energy between the two proteins. These results shed new light on the electrostatically driven mechanism governing the complex coacervation in heteroprotein systems.
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hal-04023433 , version 1 (10-03-2023)

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Rima Soussi Hachfi, Pascaline Hamon, Florence Rousseau, Marie-Hélène Famelart, Said Bouhallab. Ionic Strength Dependence of the Complex Coacervation between Lactoferrin and β-Lactoglobulin. Foods, 2023, 12, pp.1040. ⟨10.3390/foods12051040⟩. ⟨hal-04023433⟩

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