Journal Articles The Plant cell Year : 2023

Blue light promotes ascorbate synthesis by deactivating the PAS/LOV photoreceptor that inhibits GDP- l -galactose phosphorylase

Céline Bournonville
Kentaro Mori
Paul Deslous
Guillaume Decros
Jean-Philippe Mauxion
Joana Jorly
Stéphanie Gadin
Cédric Cassan
Mickael Maucourt
Daniel Just
Cécile Brès
Christophe Rothan
Carine Ferrand
Laure Bataille
Laure Beven
Pierre Pétriacq
Yves Gibon

Abstract

Abstract Ascorbate (vitamin C) is an essential antioxidant in fresh fruits and vegetables. To gain insight into the regulation of ascorbate metabolism in plants, we studied mutant tomato plants (Solanum lycopersicum) that produce ascorbate-enriched fruits. The causal mutation, identified by a mapping-by-sequencing strategy, corresponded to a knock-out recessive mutation in a class of photoreceptor named PAS/LOV protein (PLP), which acts as a negative regulator of ascorbate biosynthesis. This trait was confirmed by CRISPR/Cas9 gene editing and further found in all plant organs, including fruit that accumulated 2 to 3 times more ascorbate than in the WT. The functional characterization revealed that PLP interacted with the 2 isoforms of GDP-l-galactose phosphorylase (GGP), known as the controlling step of the l-galactose pathway of ascorbate synthesis. The interaction with GGP occurred in the cytoplasm and the nucleus, but was abolished when PLP was truncated. These results were confirmed by a synthetic approach using an animal cell system, which additionally demonstrated that blue light modulated the PLP-GGP interaction. Assays performed in vitro with heterologously expressed GGP and PLP showed that PLP is a noncompetitive inhibitor of GGP that is inactivated after blue light exposure. This discovery provides a greater understanding of the light-dependent regulation of ascorbate metabolism in plants.
Fichier principal
Vignette du fichier
2023-Bournonville-The Plant Cell.pdf (4.94 Mo) Télécharger le fichier
Origin Publisher files allowed on an open archive
Licence

Dates and versions

hal-04083263 , version 1 (27-04-2023)

Licence

Identifiers

Cite

Céline Bournonville, Kentaro Mori, Paul Deslous, Guillaume Decros, Tim Blomeier, et al.. Blue light promotes ascorbate synthesis by deactivating the PAS/LOV photoreceptor that inhibits GDP- l -galactose phosphorylase. The Plant cell, 2023, 35 (7), pp.2615-2634. ⟨10.1093/plcell/koad108⟩. ⟨hal-04083263⟩
94 View
31 Download

Altmetric

Share

More