A conformation-dependent monoclonal antibody against active chicken acetylcholinesterase - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles FEBS Letters Year : 1993

A conformation-dependent monoclonal antibody against active chicken acetylcholinesterase

Abstract

We show that the C-131 monoclonal antibody, directed against chicken AChE, recognizes active chicken AChE, but not the SDS-denatured or heat-inactivated protein. Previous results indicated that C-131 only binds to the active enzyme, and not to inactive molecules which also occur in the embryonic chicken brain. In contrast with C-131, other monoclonal antibodies obtained in the same series, such as C-6 and C-54, also recognize denatured or inactive AChE. It is noteworthy that these antibodies all seem to react with a trypsin-sensitive peptide which is present in chicken but not in mammalian or Torpedo AChE, whereas the C-131 antibody binds trypsin-modified as well as intact molecules. These results show that C-131 is highly conformation-dependent, specific for active AChE. They confirm our previous conclusion that active and inactive molecules arise from different folding processes.

Domains

Immunology

Dates and versions

hal-04083948 , version 1 (27-04-2023)

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Jean-Marc Chatel, François-Marie Vallette, Jean Massoulié, Jacques Grassi. A conformation-dependent monoclonal antibody against active chicken acetylcholinesterase. FEBS Letters, 1993, 319 (1-2), pp. 12-15. ⟨10.1016/0014-5793(93)80027-R⟩. ⟨hal-04083948⟩
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