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Journal Articles Virus Research Year : 2021

Molecular and biological characterization of a new mulberry idaeovirus

Kahraman Gürcan
  • Function : Author
Sibel Turan
  • Function : Author
Saffet Teber
  • Function : Author
İlyas Kılınçer
  • Function : Author
İbrahim Uz
  • Function : Author
Lucie Tamisier
Sebastien Massart
  • Function : Author
Kadriye Çağlayan
  • Function : Author


A virus-like disease with symptoms including mosaic structure, deformation, vein clearing and necrosis on the leaves and deformation, crumbling, and scab on the fruits was detected in black mulberry trees (Morus nigra L.) in Kayseri province of Turkey. A novel positive single‐stranded RNA virus with a bipartite genome and the mulberry badnavirus 1 (MBV-1) were detected in the black mulberry trees by high throughput sequencing and bioinformatic analyses. The novel virus RNA1 (5,796/7 nt) encodes a polyprotein (1,808 aa, 204.31 kDa) with three conserved domains, [MTR (aa 294–705), Hel (aa 971–1,226) and RdRp (aa 1,348–1,788)], whereas RNA2 (2,243 nt) encodes two putative proteins, MP (374 aa, 40.98 kDa), and CP (272 aa, 30.59 kDa), separated by an intergenic region of 97 nt. The highest amino acids identities were 70, 57 and 70 % with raspberry bushy dwarf virus (RBDV) for ORF1, MP and CP genes, respectively. The genome organization and phylogenetic analyses suggested that the novel virus is likely a putative new member of the genus Idaeovirus and it has been tentatively named black mulberry idaeovirus (BMIV). Virus survey showed both the BMIV and MBV-1 are likely prevalent in the region. Seven complete (six Turkish and one Iranian) and 41 partial genome sequences of the BMIV isolates revealed moderate genetic diversity (0.033 ± 0.001 %, 0.020 ± 0.002 % and 0.016 ± 0.002 % for RNA1, RNA2, and partial genomes, respectively). Both the BMIV and MBV-1 were detected in all tested pollens (n = 24, 100 %), in seed-borne balck mulberry saplings (n = 96, 100 %).This situation clearly revealed the potential spread risk of both viruses in black mulberry plantations and the necessity of taking precautions.
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Dates and versions

hal-04107143 , version 1 (26-05-2023)



Kahraman Gürcan, Sibel Turan, Saffet Teber, İlyas Kılınçer, İbrahim Uz, et al.. Molecular and biological characterization of a new mulberry idaeovirus. Virus Research, 2021, 298, pp.198411. ⟨10.1016/j.virusres.2021.198411⟩. ⟨hal-04107143⟩


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