Cover crops in vineyards do provide many important ecosystem functions, however wine growers are often reluctant to incorporate cover crops into their vineyard management as they are concerned about competition regarding water and nutrient availability. The objective of this study was to define the effects of three standard inter-row vegetation management strategies on vine growth and grape quality in different European regions. We hypothesize that general patterns of responses to different inter-row management can be identified across European vine growing regions independent of local climate and soil conditions. Data were collected in 2016 and 2017 in commercial vineyards located in five European temperate wine growing regions directly comparing standard inter-row vegetation management strategies in parallel in vineyards ranging from cover crops to bare ground through tillage or herbicide application. Vegetation management strongly influenced leaf chlorophyll content, shoot pruning weight and yeast assimilable nitrogen (YAN) in grape juice. Across countries, grape varieties and varying soil conditions, YAN consistently showed higher values in grapes separated by bare ground inter-rows as compared to inter-rows with a permanent vegetation cover. Other grape quality parameters, total soluble solids, total titratable acidity and berry weight were not or inconsistently affected across countries, preventing the prediction of generalized trends. We also observed higher values of soil organic matter in complete vegetation inter-rows. In conclusion, we identified general effects of inter-row vegetation management on vine vigor and grape quality across countries and grape varieties in different pedo-climatic conditions. Our study provides general response patterns as a basis for functional studies to develop local inter-row vegetation management strategies.