A new genus of stick insect from Jamaica is described, Jamanistria n. gen., which includes three species previously assigned to the genus Clonistria Stål, 1875, and one new species discovered by the authors in 2018, J. xerophila n. sp., described in both sexes and the egg. The previously unknown female and egg of Jamanistria annulipes (Rehn & Hebard, 1938) n. comb., male and egg of J. simplicitarsis (Gray, 1835) n. comb., female and egg of J. monticola (Rehn & Hebard, 1938) n. comb. are described here for the first time. A barcoding study based on COI gene of three species of the genus is presented and supports the species delimitations.