Use of two-dimensional protein-pattern analysis for the characterization of Arabidopsis thaliana mutants - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement
Journal Articles Planta Year : 1994

Use of two-dimensional protein-pattern analysis for the characterization of Arabidopsis thaliana mutants

Abstract

otal proteins extracted from wild-type plants of Arabidopsis thaliana Heyhn, an etiolated mutant, a de-etiolated mutant and a mutant affected in cotyledon morphology, were analyzed by two-dimensional gel electrophoresis. Computer analysis of two-dimensional gels allowed the characterization of the mutants by a set of proteins showing a differential expression when compared with the wild-type plant grown under the same conditions. The overlap between comparisons of the different mutants with the wild-type allowed the identification of groups of polypeptides which, since their expression is altered in several mutants, might be involved in certain physiological functions. For example, this approach showed a possible involvement of actin in the elongation process. The simultaneous analysis of the two-dimensional protein patterns of different mutants seems, therefore, to be a promising approach to characterize proteins involved in various physiological functions.
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Dates and versions

hal-04170220 , version 1 (25-07-2023)

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  • HAL Id : hal-04170220 , version 1

Cite

Veronique Santoni, Catherine Bellini, Michel Caboche. Use of two-dimensional protein-pattern analysis for the characterization of Arabidopsis thaliana mutants. Planta , 1994, 192, pp.557-566. ⟨hal-04170220⟩

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