The consumption of dairy products is often associated with a lower incidence of metabolic syndrome (MS) disorders. Minor dairy fatty acids (FA) like odd-chain FA (OCFAs, 15:0 and 17:0) and trans-palmitoleic acid (TPA) are biomarkers of dairy consumption. A rising hypothesis is that they might modulate physiological functions and could protect against MS. During 4 months, mice were fed high fat diet (HFD, 4,8 kcal/g) supplemented with either 15:0, 17:0, TPA (each at 4% energy intake) or a mix of the 3 of them (MIX diet, 1.5% of both 15:0 and 17:0 and 1% of TPA). Two control diets were implemented, a healthy control (normocaloric, 3,8 kcal/g) without FA supplementation and a MS positive control (4,8 kcal/g) supplemented with palmitic acid (4% energy). Physiological parameters were measured at the end of the experiment. All HFD mice slowly developed an obesity syndrome. Interestingly, only 17:0 and 15:0 supplemented mice’s body weights weren’t significantly increased compared to control. MIX diet mice’s subcutaneous adipose tissues weren’t significantly different from control unlike the 4 others HFD which were increased. Only HFD+C16:0 supplemented mice weren’t able to decrease their blood glucose level 2 hours after oral glucose tolerance test, compared to control. A mix of dairy FA, 15:0, 17:0 and TPA, in HFD seem to prevent some MS disorders in mice. Individually, OCFAs and TPA at 4% energy did not reduce all MS parameters, they might be efficient at smaller concentrations, as there are in dairy products.