Transcriptomic, Metabolomic and Ionomic Analyses Reveal Early Modulation of Leaf Mineral Content in Brassica napus under Mild or Severe Drought - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles International Journal of Molecular Sciences Year : 2022

Transcriptomic, Metabolomic and Ionomic Analyses Reveal Early Modulation of Leaf Mineral Content in Brassica napus under Mild or Severe Drought

Aurélien D’oria
Lun Jing
  • Function : Author
Mustapha Arkoun
  • Function : Author
Sylvain Pluchon
  • Function : Author
Stéphanie Pateyron
  • Function : Author
  • PersonId : 1219228
Institut des Sciences des Plantes de Paris-Saclay
Jacques Trouverie
CV
Philippe Etienne
Sylvain Diquélou
  • Function : Author
Alain Ourry

Abstract

While it is generally acknowledged that drought is one of the main abiotic factors affecting plant growth, how mineral nutrition is specifically and negatively affected by water deficit has received very little attention, other than being analyzed as a consequence of reduced growth. Therefore, Brassica napus plants were subjected to a gradual onset of water deficits (mild, severe, or severe extended), and leaves were analyzed at the ionomic, transcriptomic and metabolic levels. The number of Differentially Expressed Genes (DEGs) and of the most differentially accumulated metabolites increased from mild (525 DEGs, 57 metabolites) to severe (5454 DEGs, 78 metabolites) and severe extended (9346 DEGs, 95 metabolites) water deficit. Gene ontology enrichment analysis of the 11,747 DEGs identified revealed that ion transport was one of the most significant processes affected, even under mild water deficit, and this was also confirmed by the shift in ionomic composition (mostly micronutrients with a strong decrease in Mo, Fe, Zn, and Mn in leaves) that occurred well before growth reduction. The metabolomic data and most of the transcriptomic data suggested that well-known early leaf responses to drought such as phytohormone metabolism (ABA and JA), proline accumulation, and oxidative stress defense were induced later than repression of genes related to nutrient transport.

Domains

Life Sciences [q-bio]

Etienne Delannoy  : Connect in order to contact the contributor

https://hal.inrae.fr/hal-04177116

Submitted on : Friday, August 4, 2023-8:46:42 AM

Last modification on : Sunday, August 6, 2023-3:39:53 AM

Consult the full text

Dates and versions

hal-04177116 , version 1 (04-08-2023)

Identifiers

Cite

Aurélien D’oria, Lun Jing, Mustapha Arkoun, Sylvain Pluchon, Stéphanie Pateyron, et al.. Transcriptomic, Metabolomic and Ionomic Analyses Reveal Early Modulation of Leaf Mineral Content in Brassica napus under Mild or Severe Drought. International Journal of Molecular Sciences, 2022, 23 (2), pp.781. ⟨10.3390/ijms23020781⟩. ⟨hal-04177116⟩

Export

BibTeX TEI Dublin Core DC Terms EndNote Datacite

Collections

CNRS UNIV-EVRY IPS2 UNIV-PARIS-SACLAY INRAE UP-SCIENCES GS-BIOSPHERA GS-LIFE-SCIENCES-HEALTH POPS-TRANSCRIPTOMIQUE
8 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More