Transcriptomic, Metabolomic and Ionomic Analyses Reveal Early Modulation of Leaf Mineral Content in Brassica napus under Mild or Severe Drought - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue International Journal of Molecular Sciences Année : 2022

Transcriptomic, Metabolomic and Ionomic Analyses Reveal Early Modulation of Leaf Mineral Content in Brassica napus under Mild or Severe Drought

Aurélien D’oria
Lun Jing
  • Fonction : Auteur
Mustapha Arkoun
  • Fonction : Auteur
Sylvain Pluchon
  • Fonction : Auteur
Stéphanie Pateyron
  • Fonction : Auteur
  • PersonId : 1204817
Institut des Sciences des Plantes de Paris-Saclay
Jacques Trouverie
CV
Philippe Etienne
Sylvain Diquélou
  • Fonction : Auteur
Alain Ourry

Résumé

While it is generally acknowledged that drought is one of the main abiotic factors affecting plant growth, how mineral nutrition is specifically and negatively affected by water deficit has received very little attention, other than being analyzed as a consequence of reduced growth. Therefore, Brassica napus plants were subjected to a gradual onset of water deficits (mild, severe, or severe extended), and leaves were analyzed at the ionomic, transcriptomic and metabolic levels. The number of Differentially Expressed Genes (DEGs) and of the most differentially accumulated metabolites increased from mild (525 DEGs, 57 metabolites) to severe (5454 DEGs, 78 metabolites) and severe extended (9346 DEGs, 95 metabolites) water deficit. Gene ontology enrichment analysis of the 11,747 DEGs identified revealed that ion transport was one of the most significant processes affected, even under mild water deficit, and this was also confirmed by the shift in ionomic composition (mostly micronutrients with a strong decrease in Mo, Fe, Zn, and Mn in leaves) that occurred well before growth reduction. The metabolomic data and most of the transcriptomic data suggested that well-known early leaf responses to drought such as phytohormone metabolism (ABA and JA), proline accumulation, and oxidative stress defense were induced later than repression of genes related to nutrient transport.

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Sciences du Vivant [q-bio]

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https://hal.inrae.fr/hal-04177116

Soumis le : vendredi 4 août 2023-08:46:42

Dernière modification le : lundi 22 avril 2024-13:18:30

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hal-04177116 , version 1 (04-08-2023)

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Aurélien D’oria, Lun Jing, Mustapha Arkoun, Sylvain Pluchon, Stéphanie Pateyron, et al.. Transcriptomic, Metabolomic and Ionomic Analyses Reveal Early Modulation of Leaf Mineral Content in Brassica napus under Mild or Severe Drought. International Journal of Molecular Sciences, 2022, 23 (2), pp.781. ⟨10.3390/ijms23020781⟩. ⟨hal-04177116⟩

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