Hepatic Cells Derived From Induced Pluripotent Stem Cells of Pigtail Macaques Support Hepatitis C Virus Infection
Résumé
The narrow species tropism of hepatitis C virus (HCV) limits animal studies. We found that pigtail macaque (Macaca nemestrina) hepatic cells derived from induced pluripotent stem cells support the entire HCV life cycle, although infection efficiency was limited by defects in the HCV cell entry process. This block was overcome by either increasing occludin expression, complementing the cells with human CD81, or infecting them with a strain of HCV with less restricted requirements for CD81. Using this system, we can modify viral and host cell genetics to make pigtail macaques a suitable, clinically relevant model for the study of HCV infection.
Mots clés
2′C-methyl-adenosine
2′CMA
Animal Model
GLuc
Gaussia luciferase
HCV
HCVcc
HCVpp
Hepatitis C Virus
Macaca nemestrina
Macaca nemestrina induced pluripotent stem cells
Macaca nemestrina–derived hepatocyte-like cells
Mn
MnHep
MniPSC
Monkey
OCLN
Replication
VSVGpp
cell culture–derived hepatitis C virus
hepatitis C virus
hepatitis C virus pseudoparticles
occludin
vesicular stomatitis virus glycoprotein