Pesticides are present in air, water, soil and biota at concentrations sometimes higher than quality standards, proposed for protecting the environment and wildlife. In agricultural landscapes, constructed wetlands, as nature-based solutions, enable the depollution of water contaminated with nitrates and pesticides through natural processes. However, such artificial ecosystems can also act as contaminant reservoirs likely to affect biodiversity. The site making the object of this study is the constructed wetland of Rampillon (CWR, Northeast of France, Brie region) which was constructed to collect water from a 355 ha subsurface drained catchment under intensive agricultural practices. Since 2012, the site is instrumented to monitor continuously physicochemical parameters and pesticides concentrations. In addition, faunistic inventories are frequently performed within the CWR. This work aims at determining whether the CWR is viable for the aquatic wildlife and associated ecological functions through a multi -taxa, -scale, -response approach. The focus is on amphibians and benthic macroinvertebrates. The first results show: (i) concordances between period of high biological activity in amphibians and macroinvertebrates and high pesticide exposure levels, and (ii) spatial variations in litter breakdown between upstream and downstream of the CWR in relation with pesticide concentrations. In addition, biochemical and morphological biomarkers are studied in amphibians to assess their health status and to determine whether pesticides are responsible for the observed effects.