Purpose This study aimed to investigate the effects of biochar and Trichoderma spp. on soil chemical and biological properties and to determine whether their combined application could be a sustainable alternative in soil cultivated with tomatoes. We hypothesized that the application of biochar and Trichoderma spp. would alter the soil's chemical and biological properties.Methods To test this hypothesis, we conducted a field experiment and evaluated the soil's chemical and biological properties.Results Our findings revealed that seven soil parameters, namely pH, P, Na+, K+, Ca2+, Mg2+, and total organic C, had a significant influence on the activity of acid phosphatase and urease. We further used machine learning models of soil properties to predict the efficiency of biochar and Trichoderma spp. in improving soil quality. These models demonstrated satisfactory performance in simulating the changes in soil properties induced by biochar, based on both predicted and experimental yield results.Conclusion Overall, our study provides important insights into the potential use of biochar and Trichoderma spp. as sustainable alternatives to mineral fertilizer for increasing tomato yield, while also highlighting their effects on soil biological and chemical properties