Worldwide, mountain glaciers are shrinking rapidly. Consequently, large areas are becoming available for the development of novel alpine ecosystems. These harsh environments, however, delay primary succession. In this study with a local community, we conducted an inclusion experiment to investigate whether Llama glama influences soils and vegetation primary succession following glacial retreat. At the foot of the Uruashraju glacier in the Cordillera Blanca, Peru (~ 4680 m.a.s.l.), we established four llama inclusion plots and four control plots that we studied from 2019 to 2022, 24–40 years after deglacierization. After three years, the llama plots had significantly increased soil organic carbon and soil nitrogen. In the llama plots, we found a large, significant increase in vascular plant cover (+ 57%) between the second and third years of experimentation, and we identified four new species that were not present in 2019. Our results suggest that Llama glama, through their latrine behavior and role as a seed disperser, enhances the primary succession and novel ecosystem formation in recently deglacierized landscapes. Our study provides scientific support that rewilding of native Andean camelids may favor adaptation to glacier retreat and inform conservation and management strategies in proglacial landscapes.