Phenotyping of organoids: contribution of an integrated platform for their characterization
Résumé
Classical 2D in vitro cell cultures have shown their limits, and the development of "organotypic" cell cultures of epithelial tissues seems a good way to replace animal biopsies. Organoids are 3D organotypic cell cultures derived from animal tissue stem cells. Development of protocols for obtaining organoids from different organs is underway at INRAE in Jouy-en-Josas. Their characterization is an important step to verify cell differentiation. At the Animal Genetics and Integrative Biology unit (INRAE UMR1313 GABI), the Genetics, Microbiota, Health (GeMS) team has developed the production of pig intestinal organoids. The @BRIDGe (Animal Biological Resources for Integrated and Digital Genomics) platform of the GABI unit brings its expertise in histology and molecular biology for the characterization of the organoids obtained by those production protocols.