Seven amino acid types suffice to reconstruct the core fold of RNA polymerase - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement
Preprints, Working Papers, ... Year : 2021

Seven amino acid types suffice to reconstruct the core fold of RNA polymerase

Abstract

Abstract The extant complex proteins must have evolved from ancient short and simple ancestors. Nevertheless, how such prototype proteins emerged on the primitive earth remains enigmatic. The double-psi beta-barrel (DPBB) is one of the oldest protein folds and conserved in various fundamental enzymes, such as the core domain of RNA polymerase. Here, by reverse engineering a modern DPBB domain, we reconstructed its evolutionary pathway started by “interlacing homo- dimerization” of a half-size peptide, followed by gene duplication and fusion. Furthermore, by simplifying the amino acid repertoire of the peptide, we successfully created the DPBB fold with only seven amino acid types (Ala, Asp, Glu, Gly, Lys, Arg, and Val), which can be coded by only GNN and ARR (R = A or G) codons in the modern translation system. Thus, the DPBB fold could have been materialized by the early translation system and genetic code.

Dates and versions

hal-04223306 , version 1 (29-09-2023)

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Sota Yagi, Aditya Padhi, Jelena Vucinic, Sophie Barbe, Thomas Schiex, et al.. Seven amino acid types suffice to reconstruct the core fold of RNA polymerase. 2023. ⟨hal-04223306⟩
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