A new set of international Leptosphaeria maculans isolates as a resource for elucidation of the basis and evolution of blackleg disease on Brassica napus - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement
Journal Articles Plant Pathology Year : 2023

A new set of international Leptosphaeria maculans isolates as a resource for elucidation of the basis and evolution of blackleg disease on Brassica napus

Angela van de Wouw
W. Fernando
Jacqueline Batley
Alexander Idnurm

Abstract

Abstract A collection of isolates of the fungi Leptosphaeria maculans and L . biglobosa , which cause blackleg disease on Brassica napus (canola/oilseed rape) and other Brassicaceae species, was assembled to represent the global diversity of these pathogens and a resource for international research. The collection consists of 226 isolates (205 L . maculans and 21 L . biglobosa ) from 11 countries. The genomes of all 205 L . maculans isolates were sequenced, and the distribution and identity of avirulence gene alleles were determined based on genotypic information and phenotypic reactions on B . napus lines that hosted specific resistance genes. Whilst the frequencies of some avirulence alleles were consistent across each of the regions, others differed dramatically, potentially reflecting the canola/oilseed rape cultivars grown in those countries. Analyses of the single‐nucleotide polymorphism (SNP) diversity within these L . maculans isolates revealed geographical separation of the populations. This "open access" resource provides a standardized set of isolates that can be used to define the basis for how these fungal pathogens cause disease, and as a tool for discovery of new resistance traits in Brassica species.

Dates and versions

hal-04240605 , version 1 (13-10-2023)

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Angela van de Wouw, Jack Scanlan, Hawlader Al-Mamun, Marie‐hélène Balesdent, Lydia Bousset, et al.. A new set of international Leptosphaeria maculans isolates as a resource for elucidation of the basis and evolution of blackleg disease on Brassica napus. Plant Pathology, In press, ⟨10.1111/ppa.13801⟩. ⟨hal-04240605⟩
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