The Arabidopsis nitrate transporter AtNRT2.1 is targeted to the root plasma membrane - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Plant Physiology and Biochemistry Year : 2007

The Arabidopsis nitrate transporter AtNRT2.1 is targeted to the root plasma membrane

Abstract

Arabidopsis AtNRT2.1 protein is the best characterized high affinity nitrate transporter in higher plants. However, nothing is known about its sub-cellular localization. In this work, we used GFP imaging to follow the targeting of the AtNRT2.1 protein to the different cell membranes. A polyclonal antibody was also raised against a peptide derived from the AtNRT2.1 sequence. Comparison of wild type and mutant plant extracts showed that this antibody recognized specifically the AtNRT2.1 protein. Microsomal membranes were fractionated on sucrose gradients and immunological detections were performed on the different fractions. Altogether, our results demonstrate that the AtNRT2.1 protein is located in the plasma membrane of the root cells.

Dates and versions

hal-04256659 , version 1 (24-10-2023)

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Franck Chopin, Judith Wirth, Marie-France Dorbe, Laurence Lejay, Anne Krapp, et al.. The Arabidopsis nitrate transporter AtNRT2.1 is targeted to the root plasma membrane. Plant Physiology and Biochemistry, 2007, 45 (8), pp.630-635. ⟨10.1016/j.plaphy.2007.04.007⟩. ⟨hal-04256659⟩
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