A thermostable bacterial catalase-peroxidase oxidizes phenolic compounds derived from lignins - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Applied Microbiology and Biotechnology Year : 2023

A thermostable bacterial catalase-peroxidase oxidizes phenolic compounds derived from lignins

Abstract

Lignocellulosic biomass is rich in lignins, which represent a bottomless natural source of aromatic compounds. Due to the high chemical complexity of these aromatic polymers, their biological fractionation remains challenging for biorefinery. The production of aromatics from the biological valorization of lignins requires the action of ligninolytic peroxidases and laccases produced by fungi and bacteria. Therefore, identification of efficient ligninolytic enzymes with high stability represents a promising route for lignins biorefining. Our strategy consists in exploiting the enzymatic potential of the thermophilic bacterium Thermobacillus xylanilyticus to produce robust and thermostable ligninolytic enzymes. In this context, a gene encoding a putative catalase-peroxidase was identified from the bacterial genome. The present work describes the production of the recombinant protein, its biochemical characterization, and ligninolytic potential. Our results show that the catalase-peroxidase from T. xylanilyticus is thermostable and exhibits catalase-peroxidase and manganese peroxidase activities. The electrochemical characterization using intermittent pulse amperometry showed the ability of the enzyme to oxidize small aromatic compounds derived from lignins. This promising methodology allows the fast screening of the catalase-peroxidase activity towards small phenolic molecules, suggesting its potential role in lignin transformation.
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Dates and versions

hal-04288937 , version 1 (16-11-2023)

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Issa Fall, Quentin Czerwiec, Sofiene Abdellaoui, Bastien Doumèche, Marjorie Ochs, et al.. A thermostable bacterial catalase-peroxidase oxidizes phenolic compounds derived from lignins. Applied Microbiology and Biotechnology, 2023, 107 (1), pp.201-217. ⟨10.1007/s00253-022-12263-9⟩. ⟨hal-04288937⟩
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