Holistic integration of omics data reveals the drivers that shape the ecology of microbial meat spoilage scenarios - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Frontiers in Microbiology Year : 2023

Holistic integration of omics data reveals the drivers that shape the ecology of microbial meat spoilage scenarios

Simon Poirier
  • Function : Author
Gwendoline Coeuret
  • Function : Author
Marie-Hélène Desmonts
  • Function : Author
Dalal Werner
  • Function : Author
Carole Feurer
  • Function : Author
Bastien Frémaux
  • Function : Author
Sandrine Guillou
  • Function : Author
Ngoc-Du Martin Luong
  • Function : Author
Olivier Rué
Valentin Loux
  • Function : Author
Monique Zagorec
  • Function : Author
Stéphane Chaillou
  • Function : Author

Abstract

Background The use of omics data for monitoring the microbial flow of fresh meat products along a production line and the development of spoilage prediction tools from these data is a promising but challenging task. In this context, we produced a large multivariate dataset (over 600 samples) obtained on the production lines of two similar types of fresh meat products (poultry and raw pork sausages). We describe a full analysis of this dataset in order to decipher how the spoilage microbial ecology of these two similar products may be shaped differently depending on production parameter characteristics. Methods Our strategy involved a holistic approach to integrate unsupervised and supervised statistical methods on multivariate data (OTU-based microbial diversity; metabolomic data of volatile organic compounds; sensory measurements; growth parameters), and a specific selection of potential uncontrolled (initial microbiota composition) or controlled (packaging type; lactate concentration) drivers. Results Our results demonstrate that the initial microbiota, which is shown to be very different between poultry and pork sausages, has a major impact on the spoilage scenarios and on the effect that a downstream parameter such as packaging type has on the overall evolution of the microbial community. Depending on the process, we also show that specific actions on the pork meat (such as deboning and defatting) elicit specific food spoilers such as Dellaglioa algida, which becomes dominant during storage. Finally, ecological network reconstruction allowed us to map six different metabolic pathways involved in the production of volatile organic compounds involved in spoilage. We were able connect them to the different bacterial actors and to the influence of packaging type in an overall view. For instance, our results demonstrate a new role of Vibrionaceae in isopropanol production, and of Latilactobacillus fuchuensis and Lactococcus piscium in methanethiol/disylphide production. We also highlight a possible commensal behavior between Leuconostoc carnosum and Latilactobacillus curvatus around 2,3-butanediol metabolism. Conclusion We conclude that our holistic approach combined with large-scale multi-omic data was a powerful strategy to prioritize the role of production parameters, already known in the literature, that shape the evolution and/or the implementation of different meat spoilage scenarios.

Dates and versions

hal-04302764 , version 1 (23-11-2023)

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Simon Poirier, Gwendoline Coeuret, Marie-Christine Champomier-Vergès, Marie-Hélène Desmonts, Dalal Werner, et al.. Holistic integration of omics data reveals the drivers that shape the ecology of microbial meat spoilage scenarios. Frontiers in Microbiology, 2023, 14, ⟨10.3389/fmicb.2023.1286661⟩. ⟨hal-04302764⟩
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