Protection of Solanum lycopesicum induced by chitosan and chitosan nano-hydroxyapatite against Pepino mosaic virus and Verticillium dahliae - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Biocatalysis and Agricultural Biotechnology Year : 2022

Protection of Solanum lycopesicum induced by chitosan and chitosan nano-hydroxyapatite against Pepino mosaic virus and Verticillium dahliae

Nirmine Ezzouine
Rachid El Kaim Billah
  • Function : Author
Abdessadik Soufiane
Siham Esserti
  • Function : Author
Malika Belfaiza
  • Function : Author
Lalla Aicha Rifai
Kacem Makroum
  • Function : Author
Tayeb Koussa
Lydia Faize
Nuria Alburquerque
Lorenzo Burgos
Mohamed Faize

Abstract

The main of the present study was to prepare chitosan (Chit) and chitosan hydroxyapatite nanoparticle (Chit-nHap) to assess their activities against two major diseases affecting tomato crop: pepino mosaic virus (PepMV) and verticillium wilt caused by the fungus Verticillium dahliae. The synthesized compounds were characterized using X-rays diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), scanning electronic microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS). In vitro, the application of various concentrations of Chit and Chit-nHap showed significant inhibition of radial mycelial growth against V. dahliae. However, Chit-nHap was found to be more effective than Chit. In greenhouse trials, the disease severity was significantly reduced when Chit and Chit-nHap were sprayed to the leaves at 150 and 250 mu g mL- 1. The highest concentration of Chit and Chit-nHap reduced leaf alteration index by more than 81% and browning index by 96%. Both compounds also reduced PepMV disease severity and virus accumulation. Protection afforded against PepMV was associated with differential accumulation of H2O2 and with potentiation of the activity of antioxidant enzymes such as catalase, ascorbate peroxidase, guaiacol peroxidase and polyphenol oxidase.

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Biotechnology
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Dates and versions

hal-04321063 , version 1 (04-12-2023)

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Nirmine Ezzouine, Rachid El Kaim Billah, Abdessadik Soufiane, Siham Esserti, Malika Belfaiza, et al.. Protection of Solanum lycopesicum induced by chitosan and chitosan nano-hydroxyapatite against Pepino mosaic virus and Verticillium dahliae. Biocatalysis and Agricultural Biotechnology, 2022, 43, pp.102386. ⟨10.1016/j.bcab.2022.102386⟩. ⟨hal-04321063⟩
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