Association of L Protein and in vitro Tomato Spotted Wilt Virus RNA-Dependent RNA Polymerase Activity - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement
Journal Articles Intervirology Year : 2003

Association of L Protein and in vitro Tomato Spotted Wilt Virus RNA-Dependent RNA Polymerase Activity

Abstract

We have previously described an in vitro assay for RNA-dependent RNA polymerase activity in virions of tomato spotted wilt virus (TSWV). Here we report antibody inhibition of virion-associated RNA synthesis in vitro with an L-protein-specific polyclonal antibody raised against the carboxy-terminus of the L protein. In contrast, RNA synthesis was not inhibited by a heterologous antiserum and was unaffected by antiserum raised against an internal portion of the L protein. Our results directly associate the TSWV L protein, the putative viral polymerase, with RNA synthesis functions in vitro.
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Dates and versions

hal-04373207 , version 1 (04-01-2024)

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Elisabeth Chapman, Pierre Hilson, Thomas German. Association of L Protein and in vitro Tomato Spotted Wilt Virus RNA-Dependent RNA Polymerase Activity. Intervirology, 2003, 46 (3), pp.177-181. ⟨10.1159/000071459⟩. ⟨hal-04373207⟩

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