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Article Dans Une Revue Veterinary Research Année : 2019

Four types of scrapie in goats differentiated from each other and bovine spongiform encephalopathy by biochemical methods

Jan Langeveld
Laura Pirisinu
  • Fonction : Auteur
Jorg Jacobs
  • Fonction : Auteur
Maria Mazza
  • Fonction : Auteur
Isabelle Lantier
  • Fonction : Auteur
Olivier Andréoletti
Cristina Acin
  • Fonction : Auteur
Elena Esposito
  • Fonction : Auteur
Christine Fast
  • Fonction : Auteur
Martin Groschup
  • Fonction : Auteur
Wilfred Goldmann
  • Fonction : Auteur
John Spiropoulos
  • Fonction : Auteur
Theodoros Sklaviadis
  • Fonction : Auteur
Frederic Lantier
  • Fonction : Auteur
Loukia Ekateriniadou
  • Fonction : Auteur
Penelope Papasavva-Stylianou
  • Fonction : Auteur
Lucien van Keulen
  • Fonction : Auteur
Pier-Luigi Acutis
  • Fonction : Auteur
Umberto Agrimi
  • Fonction : Auteur
Alex Bossers
  • Fonction : Auteur
Romolo Nonno
  • Fonction : Auteur

Résumé

Abstract Scrapie in goats has been known since 1942, the archetype of prion diseases in which only prion protein (PrP) in misfolded state (PrP Sc ) acts as infectious agent with fatal consequence. Emergence of bovine spongiform encephalopathy (BSE) with its zoonotic behaviour and detection in goats enhanced fears that its source was located in small ruminants. However, in goats knowledge on prion strain typing is limited. A European-wide study is presented concerning the biochemical phenotypes of the protease resistant fraction of PrP Sc (PrP res ) in over thirty brain isolates from transmissible spongiform encephalopathy (TSE) affected goats collected in seven countries. Three different scrapie forms were found: classical scrapie (CS), Nor98/atypical scrapie and one case of CH1641 scrapie. In addition, CS was found in two variants—CS-1 and CS-2 (mainly Italy)—which differed in proteolytic resistance of the PrP res N - terminus . Suitable PrP res markers for discriminating CH1641 from BSE (C-type) appeared to be glycoprofile pattern, presence of two triplets instead of one, and structural (in)stability of its core amino acid region. None of the samples exhibited BSE like features. BSE and these four scrapie types, of which CS-2 is new, can be recognized in goats with combinations of a set of nine biochemical parameters.

Dates et versions

hal-04449965 , version 1 (09-02-2024)

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Citer

Jan Langeveld, Laura Pirisinu, Jorg Jacobs, Maria Mazza, Isabelle Lantier, et al.. Four types of scrapie in goats differentiated from each other and bovine spongiform encephalopathy by biochemical methods. Veterinary Research, 2019, 50 (1), pp.97. ⟨10.1186/s13567-019-0718-z⟩. ⟨hal-04449965⟩
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