Groundwater recharge is widely recognized as being the most important parameter for the sustainable management of water resources. In semiarid environments, groundwater recharge can be quantified using the piston displacement method (PDM). From a single soil sampling campaign, the PDM relies on linking the deeper vadose zone soil pore water stable isotope composition (delta H-2(H2O) and delta O-18(H2O)) to the local meteoric water line (LMWL). However, the isotopic composition of precipitation changes seasonally, influencing the water isotope composition of the vadose zone over time. Thus, it is important to test whether the PDM is sensitive to seasonal soil sampling and whether the assessed recharge rate is independent of the time of sampling. This study investigates the effect of seasonal soil sampling on the distribution of vadose zone stable isotope composition to determine whether the sampling time influences the estimate of recharge rate from PDM. Soil samples were obtained along vertical profiles through the vadose zone in a semiarid region during the spring, summer, and autumn seasons. Specifically, the delta H-2(H2O) and delta O-18(H2O) of the soil pore water were determined along vertical profiles, and the PDM was applied to quantify the annual recharge. The delta H-2(H2O) and delta O-18(H2O) values range from -7.3 to -3.5 parts per thousand and from -54.4 to +7.41 parts per thousand, respectively, and plot along a continuum with a slope less than the LMWL. Samples from deeper in the vadose zone profile had distinct ranges in isotopic composition between the three soil sampling campaigns, with isotopic composition of spring sampling dominated by lower values and those from autumn with higher values. Despite these differences, the resulting annual recharge rates from the different sampling campaigns are comparable (1.5 to 2 % of annual precipitation). Even though the pore water isotopic composition changed over time, the shift between the deeper vadose zone isotopic compositions and the LMWL remained relatively constant, leading to a similar recharge estimate over time. Therefore, the PDM-based recharge assessment in the tested semiarid environment is independent of the sampling time, which indicates that sampling for assessing groundwater recharge can be undertaken during any season.