Highlights: • Maintaining service crop till vine budburst increases biomass two to three-fold compared with early termination. • Tillage termination preserves soil water stocks and inorganic nitrogen. • Budburst and roller termination increase soil microbial activity. • Tillage termination boosts vigor, water and nitrogen status, enhancing yield. • Service crops management allows reaching satisfactory yields depending on objectives. Abstract: Soil management and particularly service crops are a promising solution for addressing current challenges in viticulture as they limit the use of herbicides while increasing potential ecosystem services. Scientific literature barely considers the importance of service crop management to reach trade-offs between ecosystem services and disservices. This study evaluates during a three-year experiment, 6 service crop termination strategies for winter service crops, combining two service crops termination periods (early termination in February vs. termination at grapevine budburst) and three termination methods (mower (M), mower + tillage (T), roller-crimper (R)). Service crop (biomass, C:N ratio, weeds, and mulch following termination), soil ( soil organic matter, microbial biomass, and water and nitrogen stocks), and grapevines (predawn leaf water potential, yield components, δ13C, yeast assimilable nitrogen in juice, and pruning weight) were monitored from 2019 to 2022. Allowing service crops to develop until the budburst of the vine resulted in a two to three-fold increase in biomass compared to early destruction. Termination involving soil tillage was the most effective method, as treatments destroyed early with soil tillage exhibited almost no regrowth. Soil tillage termination led to the lowest biomass of weeds at the grapevine flowering two years out of three, and prevented the regrowth of certain sown plants, especially Poaceae. The roller was less effective in destroying service crop species but was the best method for maintaining plant residues on the soil surface. A higher soil microbial biomass was observed with termination at budburst, combined with no-till termination methods. Termination involving soil tillage was notably more effective in stopping service crop transpiration, increasing soil water stocks and improving grapevine water status. In 2020 and 2022, soil inorganic nitrogen stocks were almost 4 times higher in the T treatments compared to the other two termination methods, achieving a level of approximately 61 kg ha−1 that closely corresponds to the annual nitrogen requirements of grapevines. The yeast-assimilable nitrogen (YAN) content in grape juice mirrored this trend. Pruning weight varied significantly between different termination methods, with T treatments exhibiting a higher pruning weight per vine in comparison to R and M treatments. In general terms, the T treatment had a significantly higher number of bunches, the M treatment had the lowest, and the R treatment exhibited intermediate values. Overall, the average grapevine yield ranged from 7.25 to 13.7 t ha−1, corresponding to 52–98 hL ha−1 (with 4000 vines ha−1, 140 kg hL−1). This level of production may be accepted for Protected Designations of Origin that limit grapevine yield to 40 or 60 hL ha−1, but it could be a limitation for Protected Geographical Indications, which permit 90 hL ha−1, or unlabeled productions without yield limitations. Given the Mediterranean climate context, with rising frequencies of dry winters due to climate change, termination involving soil tillage appears to be the least risky strategy to preserve grapevine vigor and production while improving soil-based ecosystem functions. However, this might be contingent on the targeted yield and wine valuation.