Genome-wide Translational Changes Induced by the Prion [PSI+] - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Cell Reports Year : 2014

Genome-wide Translational Changes Induced by the Prion [PSI+]

Abstract

Prions are infectious proteins that can adopt a structural conformation that is then propagated among other molecules of the same protein. [PSI(+)] is an aggregated conformation of the translational release factor eRF3. [PSI(+)] modifies cellular fitness, inducing various phenotypes depending on genetic background. However, the genes displaying [PSI(+)]-controlled expression remain unknown. We used ribosome profiling in isogenic [PSI(+)] and [psi(-)] strains to identify the changes induced by [PSI(+)]. We found 100 genes with stop codon readthrough events and showed that many stress-response genes were repressed in the presence of [PSI(+)]. Surprisingly, [PSI(+)] was also found to affect reading frame selection independently of its effect on translation termination efficiency. These results indicate that [PSI(+)] has a broader impact than initially anticipated, providing explanations for the phenotypic differences between [psi(-)] and [PSI(+)] strains.

Dates and versions

hal-04617983 , version 1 (09-07-2024)

Identifiers

Cite

Agnès Baudin-Baillieu, Rachel Legendre, Claire Kuchly, Isabelle Hatin, Stéphane Demais, et al.. Genome-wide Translational Changes Induced by the Prion [PSI+]. Cell Reports, 2014, 8 (2), pp.439-448. ⟨10.1016/j.celrep.2014.06.036⟩. ⟨hal-04617983⟩
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