Heterogeneity and Intrinsic Variation in Spatial Genome Organization - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Preprints, Working Papers, ... (Preprint) Year : 2017

Heterogeneity and Intrinsic Variation in Spatial Genome Organization


Abstract The genome is hierarchically organized in 3D space and its architecture is altered in differentiation, development and disease. Some of the general principles that determine global 3D genome organization have been established. However, the extent and nature of cell-to-cell and cell-intrinsic variability in genome architecture are poorly characterized. Here, we systematically probe the heterogeneity in genome organization in human fibroblasts by combining high-resolution Hi-C datasets and high-throughput genome imaging. Optical mapping of several hundred genome interaction pairs at the single cell level demonstrates low steady-state frequencies of colocalization in the population and independent behavior of individual alleles in single nuclei. Association frequencies are determined by genomic distance, higher-order chromatin architecture and chromatin environment. These observations reveal extensive variability and heterogeneity in genome organization at the level of single cells and alleles and they demonstrate the coexistence of a broad spectrum of chromatin and genome conformations in a cell population.
Fichier principal
Vignette du fichier
171801v2.full.pdf (420.22 Ko) Télécharger le fichier
Origin Files produced by the author(s)

Dates and versions

hal-04641845 , version 1 (09-07-2024)



Elizabeth Finn, Gianluca Pegoraro, Hugo Brandão, Anne-Laure Valton, Marlies Oomen, et al.. Heterogeneity and Intrinsic Variation in Spatial Genome Organization. 2024. ⟨hal-04641845⟩
0 View
0 Download



Gmail Mastodon Facebook X LinkedIn More