Genome-wide association study of the rabbit caecal microbiota composition
ANALYSE GENOMIQUE DE LA COMPOSITION DU MICROBIOTE CAECAL DU LAPIN
Résumé
The aim of our study was to identify genomic regions influencing the rabbit caecal microbiota composition for a better knowledge of the genetic determinism of this trait. The ribosomal 16S DNA was extracted from the microbial community of the caecum and then sequenced in 525 rabbits originated either from a line selected on feed efficiency or a control non-selected line. After rarefaction and filtration, the sequences were used to identify 859 OTUs (operational taxonomic units) or phylotypes. The rabbits were genotyped with the Affimetrix Axiom Rabbit 200K chip. After quality control 514 rabbits and 103,072 SNPs were retained for association study analyses. Eighty-one QTLs were identified for 69 OTUs’ abundance with a total of 492 significant SNPs (P< 0.05 genome-wide). Most QTLs were associated with the abundance of a single OTU. Eight QTLs were associated with 2 or 3 OTUs. Candidate genes related to immune response or bile acid metabolism in these regions are discussed.
| Origine | Fichiers produits par l'(les) auteur(s) |
|---|