Identification of a "flavescence dorée" phytoplasma VmpA candidate receptor in the experimental insect vector Euscelidius variegatus - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Conference Papers Year : 2023

Identification of a "flavescence dorée" phytoplasma VmpA candidate receptor in the experimental insect vector Euscelidius variegatus

Francesca Canuto
  • Function : Speaker
  • PersonId : 1334830
  • IdRef : 274471418
Sybille Duret
  • Function : Author
  • PersonId : 1206847
Marie-Pierre Dubrana-Ourabah
  • Function : Author
  • PersonId : 1206848
Laure Beven
Christophe Garcion
Lysiane Brocard
Stéphane Claverol
Xavier Foissac

Abstract

The "flavescence dorée" phytoplasma initiates the infection process using the adhesin VmpA to adhere to insect vector cells although the insect receptor for this protein still remains unknown. In vitro interaction assays were performed using recombinant VmpA-His 6 and cultured cells of the experimental vector Euscelidius variegatus. It was possible to identify thirteen interesting putative VmpA targets that have been tested for their role as VmpA receptors in cultured cells subjected to RNAi. These preliminary results show the implication of an unidentified protein containing several leucine-rich repeat domains in the adhesion of beads coated with recombinant VmpA to vector cells in culture.

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Dates and versions

hal-04679576 , version 1 (28-08-2024)

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  • HAL Id : hal-04679576 , version 1

Cite

Francesca Canuto, Nathalie Arricau-Bouvery, Sybille Duret, Marie-Pierre Dubrana-Ourabah, Laure Beven, et al.. Identification of a "flavescence dorée" phytoplasma VmpA candidate receptor in the experimental insect vector Euscelidius variegatus. 5th Meeting of the International Phytoplasmologist Working Group, May 2023, Muscat, Oman. ⟨hal-04679576⟩
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