Tnt1 retrotransposon as an efficient tool for development of an insertional mutant collection of Lotus japonicus - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles In Vitro Cellular & Developmental Biology - Plant Year : 2016

Tnt1 retrotransposon as an efficient tool for development of an insertional mutant collection of Lotus japonicus

Abstract

The Tnt1 retrotransposon of tobacco (Nicotiana tabacum) has proven to be a very efficient mutagen for the model legume Medicago truncatula ecotype 108 and cultivar Jemalong 2HA and for economically important plants, such as soybean and potato. In this study, the activity of Tnt1 in the model legume Lotus japonicus L. was tested. First, a new regeneration and transformation protocol was developed for L. japonicus that represents a new tool for legume mutagenesis and reverse genetics. Using this protocol, the Tnt1 retrotransposon was introduced into L. japonicus by Agrobacterium tumefaciens-mediated transformation, and primary transgenic lines, named starter lines, were constructed. In vitro regeneration via indirect somatic embryogenesis using starter lines harboring two to eight copies of the transgene resulted in new Tnt1 transposition events. The Tnt1 retrotransposon remained inactive during plant growth and in the T-1 progeny, indicating that it is well suited for insertional mutagenesis in L. japonicus.
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Dates and versions

hal-02635064 , version 1 (27-05-2020)

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Anelia Iantcheva, Miglena Revalska, Grigor Zehirov, Irina Boycheva, Kevin Magne, et al.. Tnt1 retrotransposon as an efficient tool for development of an insertional mutant collection of Lotus japonicus. In Vitro Cellular & Developmental Biology - Plant, 2016, 52 (3), pp.338-347. ⟨10.1007/s11627-016-9768-3⟩. ⟨hal-02635064⟩
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