Dissolved organic matter (DOM) is a complex matrix of organic materials obtained from water filtration (typically from 0.7μm). Playing a key role as an interaction between biological and geochemical process, DOM is directly involved in carbon transfer but also in the microbial growth and activities as a source of nutrients and energy. In aquatic environments, DOM can originate from both autochthonous sources such as microorganisms lysis and roots exudates, and allochtonous sources through point discharges (discharges from wastewater treatment plants, storm overflows and industrial discharges) and diffuse discharges (soil leaching and urban runoff). At a scale of a suburban watershed, anthropogenic activities coupled with landuses and occupations increase the diversity of DOM sources. Point sources can readily be identified and sampled unlike diffuse sources. Indeed, during runoffs, the accumulation of inputs, the changes relative to DOM transport as well as biological degradation processes makes it difficult to identify and trace diffuse sources of DOM in water. Thus, better identification of diffuse sources can facilitate the identification of anthropogenic discharges in watercourses. In this context, this study aims to understand the transfer of the DOM fingerprint between the terrestrial and aquatic ecosystem through the identification of reliable and specific physico-chemical and molecular markers from specific diffuse terrigenous sources. In the first stage, water and adjacent soil samples characterized by forest and wine-growing occupations were collected. Then, a leaching simulation and a biodegradation study were carried out on the soil samples. Finally, a multi-analytical approach (UV-Vis spectroscopic analysis, 3D fluorescence, stearic exclusion chromatography and high-resolution mass spectrometry analysis) was applied to water and soil leachate samples to identify and compare specific conservative markers. The spectral signatures obtained from the UV-vis data show an evolution of the quality of soil leachate organic matter as a function of the degradation time which tends towards the signatures of the MOD sampled from the adjacent watercourses. This tendency remains to be confirmed with other optical and molecular data.