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Contribution de l'approche effect directed analysis à l'identification de perturbateurs endocriniens dans les milieux aquatiques

Abstract : The aim of this thesis was to develop and to evaluate an effect directed analysis (EDA) approach, combining in vitro bioassays and chemical analysis for the characterization of aquatic systems contamination by endocrine disrupting chemicals (EDCs) and their identification. For integrative contamination assessment, new in vitro tools have been developed and evaluated for the detection of emerging pollutants, and then integrated in a multi-bioassays approach. In particular, the human pregnane X receptor (PXR) based bioassay provided further information on bioactive contaminants and thereby enhanced the environmental diagnostic as regard to classical biological target (e.g. estrogen (ER), androgen (AR) or aryl hydrocarbon receptors (AhR)). Such multi-receptors approach allowed determining novel integrative toxicity profiles in sediment and surface water. Overall, our results demonstrated the need to integrate other biological target than ER and AhR, and to assess contamination partitioning for a better hazard/risk assessment. For such purpose, combined use of polar organic compound integrative sampler and in vitro bioassays provided suitable tools to assess active hydrosoluble chemicals in surface water. Finally, in order to identify the compounds responsible for the detected activities, the multi-receptors approach has been integrated in EDA strategy applied to two river sites under different chemical pressures (urban/agricultural and pharmaceutical). To tackle this challenge, extraction and fractionation methods were developed and evaluated in order to encompass a diversity of active chemicals that are detected by in vitro bioassays. The EDA approach allowed determining partial contribution of priority pollutants in biological responses as well as the identification of new EDCs.
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https://hal.inrae.fr/tel-02980485
Contributor : Nicolas Creusot <>
Submitted on : Monday, November 16, 2020 - 2:44:35 PM
Last modification on : Wednesday, March 17, 2021 - 3:33:06 AM

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Nicolas Creusot. Contribution de l'approche effect directed analysis à l'identification de perturbateurs endocriniens dans les milieux aquatiques. Chimie analytique. Université de Bordeaux 1, 2011. Français. ⟨tel-02980485⟩

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