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Contribution à l'étude de l'expression des gènes de végétaux supérieurs. Analyse des ARNm de racines de Fève et de différentes gliadines du Blé

Abstract : With the purpose of studying the extent of gene expression during cell differentiation, we have analysed diverse mRNA populations. First the polysomal polyadenylated RNA populations of meristematic, elongating and mature root cells, were analysed and compared by performing homologous and heterologous hybridization kinetics beetwen cDNA and mRNA. The three populations are distributed in three abundance classes (abundant, intermediate and rare). The comparison of the three phenotypes shows that a few abundant sequences can be regarded as meristematic-stage specific. Furthemore, cell elongation is accompagnied by the appearance of new transcripts belonging to the intermediate and rare classes. The same approach was used to investigate the complexity of mRNA isolated from the wheat endosperm. We demonstrate that 60% of the mRNA molecules being translated in the endosperm, corresponding to less than 1% of total diverse sequences, contain the information for approximately 75 superabundant species which would encode most of the seed storage proteins especially gliadins. We have cloned double stranded cDNA corresponding to these sequences and characterized several clones representing each of the three gliadins sub-families. Such clones can be used to determine the amino acid sequence and the secondary structure of these proteins. cDNA probes representing the major gliadins were used to follow the expression of corresponding genes during the development of the wheat seed. The gliadins structural genes will be a valuable substrate for studying the regulatory sequences involved in the control of such a multigenic family activity.
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Habilitation à diriger des recherches
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https://hal.inrae.fr/tel-03160025
Contributor : Victor Vaillant <>
Submitted on : Thursday, March 4, 2021 - 10:29:22 PM
Last modification on : Thursday, May 27, 2021 - 3:35:30 AM
Long-term archiving on: : Saturday, June 5, 2021 - 7:26:51 PM

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Victor Vaillant. Contribution à l'étude de l'expression des gènes de végétaux supérieurs. Analyse des ARNm de racines de Fève et de différentes gliadines du Blé. Sciences du Vivant [q-bio]. Université Paris Diderot - Paris 7, 1986. ⟨tel-03160025⟩

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