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Analyse fonctionnelle du gene vestigial chez drosophila melanogaster

Abstract : Drosophila has become a model organism for the study of genes involved in the formation of appendages (wings and legs). These same genes often perform similar functions in the formation of limbs in vertebrae. They are at the center of a cascade of genetic regulations necessary for the formation of cell compartments which allow limb growth. We were able to demonstrate that vestigial interacts at the protein level with the scalloped protein (sd). This protein is part of a family of transcription factors conserved from yeast to humans. We were able to show that in vitro and in vivo, the sd / vg complex formed a bipartite transcription factor which binds to DNA via the sd protein and activates transcription via vg. We have also identified a new human gene, called tondu (tdu), which encodes a protein with sequence similarities to the vg protein. We have shown that this protein interacts with tefs transcription factors, homologous to the sd protein in mammals, as well as with the sd protein itself. We have also shown that tdu can substitute for vg during the development of Drosophila. The continuation of this project requires to determine the function of tdu in mammals. In this context, it will be necessary to specify in which structures the shorn gene is expressed during development in mice. It will also be interesting to identify other partners associating with the tdu / tef and vg / sd complexes in order to better understand at the molecular level the function of these transcription factors in Drosophila and in mammals.
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https://hal.inrae.fr/tel-03179926
Contributor : Pascal Vaudin <>
Submitted on : Wednesday, March 24, 2021 - 3:55:20 PM
Last modification on : Monday, April 12, 2021 - 9:50:03 AM

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  • HAL Id : tel-03179926, version 1

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Pascal Vaudin. Analyse fonctionnelle du gene vestigial chez drosophila melanogaster. Biologie du développement. Université Paris 6 (UPMC), 2000. Français. ⟨tel-03179926⟩

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