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Theses

Vaccination à ADN intranasale sur un modèle porcin.

Abstract : Vaccination is one of the most powerful preventive strategy for controlling many infections caused by pathogens. The main route of entry for pathogens are mucosal surfaces. Most vaccines are predominantly inoculated parenterally and are generally capable of inducing protective immune responses, but not always. It is important to have new alternative strategies such as mucosal vaccines. However, the immunogenicity of such mucosal subunit vaccines (protein or plasmid DNA) is often insufficient in rents animals and in humans, so improvements are needed. In order to assess the feasibility of such a strategy in pigs, we used a plasmid DNA vaccine encoding the porcine pseudorabies virus (PrV) glycoprotein B (gB) known to be very highly immunogenic. This plasmid was or was not associated with four nanovectors described for the transport of plasmid DNA in vivo at mucosal sites (chitosan, mannosylated chitosan, KLN47 and PLGA-PEI) and was inoculated intranasally in pigs. Unexpectedly, the naked DNA vaccine induced stronger immune responses than those obtained for the four formulated DNA vaccines. However, it appeared that two of the formulations evaluated, PLGA-PEI and KLN47, appear to have stronger immunizing power. Several strategies for improving the vaccine composition and the inoculation protocol are discussed at the end of the manuscript.
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https://hal.inrae.fr/tel-03313764
Contributor : Laurent Souci <>
Submitted on : Wednesday, August 4, 2021 - 4:01:06 PM
Last modification on : Thursday, August 5, 2021 - 3:37:13 AM

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  • HAL Id : tel-03313764, version 1

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Laurent Souci. Vaccination à ADN intranasale sur un modèle porcin.. Sciences du Vivant [q-bio]. Université Rennes 1, 2020. Français. ⟨tel-03313764⟩

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