Detection and quantification of boscalid and its metabolites in honeybees - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Chemosphere Year : 2016

Detection and quantification of boscalid and its metabolites in honeybees


Boscalid is a new-generation fungicide that has been detected in several bee matrices. The objective of this work was to characterize boscalid metabolites in honeybees based on in vivo experimentation, and next to verify the presence of theses metabolites into honeybees from colonies presenting troubles. A methodology based on complementary mass spectrometric tools, namely ultra-high performance liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-QToF) or triple quadrupole mass spectrometry (UHPLC-QqQ) was implemented. Honeybees were sprayed with boscalid, at field rate (to induce the metabolization process) and the parent compound with its generated metabolites were then extracted using modified EU-QuEChERS method. The mass characteristics including exact mass, isotopic profile and mass fragments allowed assuming the structure of several metabolites. Some of them were unambiguously identified by comparison with synthesized analytical standards. The metabolites were resulted from hydroxylation and dechlorination of the parent compound as well as the substitution of a chlorine atom with an hydroxyl group. The metabolites were then quantified in bee samples collected from various beehives located in France. Boscalid and three of its metabolites were present in some samples at a level ranged between 0.2 and 36.3 ng/g.
Fichier principal
Vignette du fichier
2016 -Publ11-Jabot-Tchamitchian-Belzunces-Chemosphere_1.pdf (558.49 Ko) Télécharger le fichier
Origin : Files produced by the author(s)

Dates and versions

hal-01355547 , version 1 (28-05-2020)



Claire Jabot, Gaëlle Daniele, Barbara Giroud, Sylvie Tchamitchian, Luc P Belzunces, et al.. Detection and quantification of boscalid and its metabolites in honeybees. Chemosphere, 2016, 156, pp.245-251. ⟨10.1016/j.chemosphere.2016.04.135⟩. ⟨hal-01355547⟩
195 View
479 Download



Gmail Facebook Twitter LinkedIn More