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Molecular evidence that follicle development is accelerated in vitro compared to in vivo

Abstract : In this study, we systematically compared the morphological, functional and molecular characteristics of granulosa cells and oocytes obtained by a three-dimensional in vitro model of ovine ovarian follicular growth with those of follicles recovered in vivo. Preantral follicles of 200 mu m diameter were recovered and cultured up to 950 mu m over a 20-day period. Compared with in vivo follicles, the in vitro culture conditions maintained follicle survival, with no difference in the rate of atresia. However, the in vitro conditions induced a slight decrease in oocyte growth rate, delayed antrum formation and increased granulosa cell proliferation rate, accompanied by an increase and decrease in CCND2 and CDKN1A mRNA expression respectively. These changes were associated with advanced granulosa cell differentiation in early antral follicles larger than 400 mu m diameter, regardless of the presence or absence of FSH, as indicated by an increase in estradiol secretion, together with decreased AMH secretion and expression, as well as increased expression of GJA1, CYP19A1, ESR1, ESR2, FSHR, INHA, INHBA, INHBB and FST. There was a decrease in the expression of oocyte-specific molecular markers GJA4, KIT, ZP3, WEE2 and BMP15 in vitro compared to that in vivo. Moreover, a higher percentage of the oocytes recovered from cultured follicles 550 to 950 mu m in diameter was able to reach the metaphase II meiosis stage. Overall, this in vitro model of ovarian follicle development is characterized by accelerated follicular maturation, associated with improved developmental competence of the oocyte, compared to follicles recovered in vivo.
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https://hal.archives-ouvertes.fr/hal-01606229
Déposant : Archive Ouverte Prodinra <>
Soumis le : lundi 2 octobre 2017 - 22:31:01
Dernière modification le : mardi 26 mai 2020 - 11:06:06

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Véronique Cadoret, Cynthia Frapsauce, Peggy Jarrier-Gaillard, Virginie Maillard, Agnès Bonnet, et al.. Molecular evidence that follicle development is accelerated in vitro compared to in vivo. Reproduction, BioScientifica, 2017, 153 (5), pp.493-508. ⟨10.1530/REP-16-0627⟩. ⟨hal-01606229⟩

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