Technical note: High-throughput method for antifungal activity screening in a cheese-mimicking model - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Journal of Dairy Science Year : 2018

Technical note: High-throughput method for antifungal activity screening in a cheese-mimicking model

Abstract

In this study, we developed a high-throughput antifungalactivity screening method using a cheese-mimicking matrix distributed in 24-well plates. This method allowed rapid screening of a large variety of antifungal agent candidates: bacterial fermented ingredients, bacterial isolates, and preservatives. Using the proposed method, we characterized the antifungal activity of 44 lactic acid bacteria (LAB) fermented milk-based ingredients and 23 LAB isolates used as protective cultures against 4 fungal targets (Mucor racemosus, Penicillium commune, Galactomyces geotrichum, and Yarrowia lipolytica). We also used this method to determine the minimum inhibitory concentration of a preservative, natamycin, against 9 fungal targets. The results underlined the strain-dependency of LAB antifungal activity, the strong effect of fermentation substrate on this activity, and the effect of the screening medium on natamycin minimum inhibitory concentration. Our method could achieved a screening rate of 1,600 assays per week and can be implemented to evaluate antifungal activity of microorganisms, fermentation products, or purified compounds compatible with dairy technology.

Dates and versions

hal-01773147 , version 1 (20-04-2018)

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Lucille Garnier, Marcia Leyva Salas, Nicolas Pinon, Norman Wiernasz, Audrey Pawtowski, et al.. Technical note: High-throughput method for antifungal activity screening in a cheese-mimicking model. Journal of Dairy Science, 2018, 101 (6), ⟨10.3168/jds.2017-13518⟩. ⟨hal-01773147⟩
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