Seed comparative genomics in three coffee species identify desiccation tolerance mechanisms in intermediate seeds - Archive ouverte HAL Access content directly
Journal Articles Journal of Experimental Botany Year : 2020

Seed comparative genomics in three coffee species identify desiccation tolerance mechanisms in intermediate seeds

(1) , (1) , (1) , (2) , (3) , (4) , (1) , (3) , (5) , (1) , (1)
1
2
3
4
5

Abstract

In contrast to desiccation-tolerant 'orthodox' seeds, so-called 'intermediate' seeds cannot survive complete drying and are short-lived. All species of the genus Coffea produce intermediate seeds, but they show a considerable variability in seed desiccation tolerance (DT), which may help to decipher the molecular basis of seed DT in plants. We performed a comparative transcriptome analysis of developing seeds in three coffee species with contrasting desiccation tolerance. Seeds of all species shared a major transcriptional switch during late maturation that governs a general slowdown of metabolism. However, numerous key stress-related genes, including those coding for the late embryogenesis abundant protein EM6 and the osmosensitive calcium channel ERD4, were up-regulated during DT acquisition in the two species with high seed DT, C. arabica and C. eugenioides. By contrast, we detected up-regulation of numerous genes involved in the metabolism, transport, and perception of auxin in C. canephora seeds with low DT. Moreover, species with high DT showed a stronger down-regulation of the mitochondrial machinery dedicated to the tricarboxylic acid cycle and oxidative phosphorylation. Accordingly, respiration measurements during seed dehydration demonstrated that intermediate seeds with the highest DT are better prepared to cease respiration and avoid oxidative stresses.
Fichier principal
Vignette du fichier
erz508.pdf (1.35 Mo) Télécharger le fichier
Origin : Publication funded by an institution
Loading...

Dates and versions

hal-02403786 , version 1 (11-12-2019)

Licence

Attribution - NonCommercial - CC BY 4.0

Identifiers

Cite

Anna K Stavrinides, Stéphane Dussert, Marie-Christine Combes, Isabelle Fock-Bastide, Dany Severac, et al.. Seed comparative genomics in three coffee species identify desiccation tolerance mechanisms in intermediate seeds. Journal of Experimental Botany, 2020, 71 (4), pp.1-16/erz508. ⟨10.1093/jxb/erz508⟩. ⟨hal-02403786⟩
173 View
141 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More