Nanopore adaptive sampling to identify the NLR-gene family in melon (Cucumis melo L.) - Unité Génétique et Amélioration des Fruits et Légumes _ INRAE
Pré-Publication, Document De Travail Année : 2023

Nanopore adaptive sampling to identify the NLR-gene family in melon (Cucumis melo L.)

Résumé

Nanopore Adaptive Sampling (NAS) offers a promising approach for assessing genetic diversity in targeted genomic regions. Herein, we design and validate an experiment to enrich a set of resistance genes in several melon cultivars as a proof of concept. We showed that each of the 15 regions we identified in two newly assembled melon genomes (subspecies melo) were successfully and accurately reconstructed as well as in a third cultivar from the agrestis subspecies. We obtained a fourfold enrichment, independently from the samples, but with some variations according to the enriched regions. In the agrestis cultivar, we further confirmed our assembly by PCR. We discussed parameters that can influence enrichment and accuracy of assemblies generated through NAS. Altogether, we demonstrated NAS as a simple and efficient approach to explore complex genomic regions. This approach finally unlocks the characterization of resistance genes for a large number of individuals, as required for breeding new cultivars responding to the agroecological transition.
Fichier principal
Vignette du fichier
Belinchon_et_al-bioRxiv-2023.pdf (3.1 Mo) Télécharger le fichier
Origine Fichiers produits par l'(les) auteur(s)
licence

Dates et versions

hal-04670606 , version 1 (28-08-2024)

Licence

Identifiants

Citer

Javier Belinchon-Moreno, Aurelie Berard, Aurelie Canaguier, Veronique Chovelon, Corinne Cruaud, et al.. Nanopore adaptive sampling to identify the NLR-gene family in melon (Cucumis melo L.). 2024. ⟨hal-04670606⟩
84 Consultations
5 Téléchargements

Altmetric

Partager

More