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Article dans une revue

Wheat chromatin architecture is organized in genome territories and transcription factories

Abstract : BACKGROUND: Polyploidy is ubiquitous in eukaryotic plant and fungal lineages, and it leads to the co-existence of several copies of similar or related genomes in one nucleus. In plants, polyploidy is considered a major factor in successful domestication. However, polyploidy challenges chromosome folding architecture in the nucleus to establish functional structures. RESULTS: We examine the hexaploid wheat nuclear architecture by integrating RNA-seq, ChIP-seq, ATAC-seq, Hi-C, and Hi-ChIP data. Our results highlight the presence of three levels of large-scale spatial organization: the arrangement into genome territories, the diametrical separation between facultative and constitutive heterochromatin, and the organization of RNA polymerase II around transcription factories. We demonstrate the micro-compartmentalization of transcriptionally active genes determined by physical interactions between genes with specific euchromatic histone modifications. Both intra- and interchromosomal RNA polymerase-associated contacts involve multiple genes displaying similar expression levels. CONCLUSIONS: Our results provide new insights into the physical chromosome organization of a polyploid genome, as well as on the relationship between epigenetic marks and chromosome conformation to determine a 3D spatial organization of gene expression, a key factor governing gene transcription in polyploids.
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Déposant : Christine Molé <>
Soumis le : mardi 12 mai 2020 - 17:48:07
Dernière modification le : mercredi 1 juillet 2020 - 12:14:03


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Lorenzo Concia, Alaguraj Veluchamy, Juan Ramirez-Prado, Azahara Martin-Ramirez, Ying Huang, et al.. Wheat chromatin architecture is organized in genome territories and transcription factories. Genome Biology, BioMed Central, 2020, 21 (1), pp.1-20. ⟨10.1186/s13059-020-01998-1⟩. ⟨hal-02571325⟩



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