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Poster communications

Development of q-PCR approaches to assess water quality: Effects of cadmium and pesticides on gene expression of diatoms

Abstract : Periphytic diatoms are valuable tools to determine freshwater rivers quality based on diatoms identifications and distribution and constitute key indices in international standards. Nevertheless, indices currently used for water quality assessment do not take in consideration the diverse nature of contaminants, are time consuming and require important taxonomic knowledge. In this context development of q-PCR approaches have been developed to enhance a better reactivity in the diagnosis of modifications due to pollution conditions and to understand the genetic impact in relation with diatoms species. Through different laboratory experiments (14 days exposure) concerning various contamination (metallic with Cd: 10 and 100 µg/L) or pesticides with diuron: 1 and 10 µg/L), several target genes involved in mitochondrial metabolism (coxI, nad4, 12S), oxidative stress response (sod Mn), detoxification (cyp1A1) and photosynthesis (psaA, d1) have been characterized and their expression levels have been determined.
Mots-clés : DIURON
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Poster communications
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https://hal.inrae.fr/hal-02599228
Contributor : Migration Irstea Publications <>
Submitted on : Saturday, May 16, 2020 - 2:04:20 AM
Last modification on : Thursday, June 3, 2021 - 5:34:03 PM

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  • HAL Id : hal-02599228, version 1
  • IRSTEA : PUB00039842

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S. Kim Tiam, S. Moisset, A. Feurtet Mazel, Nicolas Mazzella, A. Arini, et al.. Development of q-PCR approaches to assess water quality: Effects of cadmium and pesticides on gene expression of diatoms. SETAC North America, Nov 2013, Nashville, United States. pp.1, 2013. ⟨hal-02599228⟩

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